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在線閱讀 --自然科學版 2020年6期《真蘚甲醇提取物對腫瘤細胞體外生長的影響》
真蘚甲醇提取物對腫瘤細胞體外生長的影響--[在線閱讀]
汪琛穎1, 李萍1, 李閏婷2, 馮若冰1, 李丹婷1, 李昊1, 張瓊丹1
1. 鄭州師范學院 生命科學學院, 河南 鄭州 450044;
2. 鄭州師范學院 分子生物學實驗室, 河南 鄭州 450044
起止頁碼: 526--530頁
DOI: 10.13763/j.cnki.jhebnu.nse.2020.06.011
摘要
利用AlamarBlue法對不同來源的真蘚(Bryum argenteum Hedw.)甲醇提取物在體外分別對4種腫瘤細胞的增殖抑制作用進行評價.結果表明:不同來源的真蘚甲醇提取物對于同種及不同種腫瘤細胞活性的影響不同.野外采集的真蘚植株、改良Knop液體培養的真蘚原絲體的甲醇提取物對人急性淋巴白血病細胞株Nalm 6和人胚腎細胞HEK293T的生長均表現抑制作用.2種來源的真蘚甲醇提取物對HEK293T作用的EC50值分別是15.12,2.14mg/mL,對Nalm 6作用的EC50值分別是1.19,5.68mg/mL,對人食管鱗癌KYSE450細胞的增殖無抑制作用.

Effects of Methanol Extract from Bryum argenteum Hedw. on the Growth of Tumor Cells in Vitro
WANG Chenying1, LI Ping1, LI Runting2, FENG Ruobing1, LI Danting1, LI Hao1, ZHANG Qiongdan1
1. School of Life Science, Zhengzhou Normal University, Henan Zhengzhou 450044, China;
2. Laboratory of Molecular Biology, Zhengzhou Normal University, Henan Zhengzhou 450044, China
Abstract:
The AlamarBlue assay was used to evaluate the inhibitory effect of methanol extracts of Bryum argenteum Hedw.from different sources on the proliferation of four tumor cells in vitro.The results showed that the methanol extracts of B.argenteum from different sources had diverse effects on the proliferation of the same and different tumor cells.The growth of human acute lymphocytic leukemia cell line Nalm-6 and human embryonic kidney cell line HEK293T were inhibited by the methanol extracts from the gametophyte collected in the field and the protonema of B.argenteum cultured in the improved Knop liquid.The EC50 values of the two kinds of B.argenteum methanol extracts on HEK293T were 15.12,2.14mg/mL,respectively,and the EC50 on Nalm-6 were 1.19,5.68mg/mL,respectively.No inhibitory effect was observed on the proliferation of human esophageal squamous cell carcinoma KYSE450 cells.

收稿日期: 2020-04-05
基金項目: 河南省本科高校省級大學生創新訓練計劃項目(S201912949005);鄭州師范學院大學生創新計劃項目(DCZ2018007);鄭州師范學院教師教育課程改革研究項目(2019-ZSJS072Z)

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